CULTURE OF CARP HEMATOPOIETIC-CELLS

Carp (cyprinus carpio) hematopoietic cells collected from the kidney-h ematopoietic tissue were cultured. After 3-5 days, colonies consisting of a few to 30 small round cells were observed. The number of cells i n the colonies increased steadily. Colonies having several hundreds of cells were observed later. Colonnies were formed on an adherent cell layer, which is possibly acting as feeder cells for the colony. Most c ells of the adherent layer were 0.1-1.0 mm in diameter and contained 3 -200 oval or round nuclei, similar in appearance to that of multinucle ated giant cells. The proliferated cells in the colonies were separate d from the colony into the culture medium. These floating cells were c onstantly produced for more than 1 month while half of the medium was renewed at 1-5 day intervals. Morphologically, the majority of the flo ating cells had features of immature type of cells which were mainly r ound cells with basophilic cytoplasm and a round nucleus. Mitotic figu res were frequently observed.