FINE MAPPING OF THE 1Q21 BREAKPOINT OF THE PAPILLARY VENAL CELL CARCINOMA-ASSOCIATED (X-1) TRANSLOCATION

A combination of Southern blot analysis on a panel of tumor-derived so matic cell hybrids and fluorescence in situ hybridization (FISH) techn iques was used to map a series of DNA markers relative to the 1q21 bre akpoint of the renal cell carcinoma (RCC)-associated (X;1)-(p11;q21) t ranslocation. This breakpoint maps between several members of the S100 family which are clustered in the 1q21 region and a conserved region between man and mouse containing the markers SPTA1-CRP-APCS-FcER1A-ATP 1A2-APOA2. The location of the breakpoint coincides with the transitio n of a region of synteny of human chromosome 1 with mouse chromosomes 3 and 1.