SIZE-EXCLUSION LIQUID-CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS OFPOLLEN ALLERGENS

Allergens from the pollen of Phleum pratense, Dactylis glomerata. Arrh enatherum elatius, Secale cereale, Lolium perrene and Festuca sp. were analysed by size-exclusion chromatography (SEC) and capillary electro phoresis (CE). SEC was used for the determination of the molecular mas ses of main allergens. A CE method, using either 150 mmol/l phosphoric acid (pH 1.8) or a micellar system consisting of 50 mmol/l sodium dod ecyl sulphate-20 mmol/l berate (pH 9.35), was developed as a rapid and efficient alternative to SEC, especially for process control of aller genic preparations. The results obtained by the two methods confirmed similarities in the structures of the studied pollen allergens.