SEQUENTIAL ACTIONS OF GLUCOSE-OXIDASE AND PEROXIDASE IN MOLECULAR FILMS ASSEMBLED BY LAYER-BY-LAYER ALTERNATE ADSORPTION

Authors
ONDA M LVOV Y ARIGA K KUNITAKE T
Citation
M. Onda et al., SEQUENTIAL ACTIONS OF GLUCOSE-OXIDASE AND PEROXIDASE IN MOLECULAR FILMS ASSEMBLED BY LAYER-BY-LAYER ALTERNATE ADSORPTION, Biotechnology and bioengineering, 51(2), 1996, pp. 163-167
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology and bioengineering → ACNP
ISSN journal
00063592
Volume
51
Issue
2
Year of publication
1996
Pages
163 - 167
Database
ISI
SICI code
0006-3592(1996)51:2<163:SAOGAP>2.0.ZU;2-R
Abstract
Molecular films of protein/polyion layers were assembled by means of a lternate adsorption through electrostatic interaction. Glucose oxidase (GOD)and peroxidase (POD) were assembled in combination with sodium p oly(styrenesulfonate) (PSS) and poly(ethyleneimine) (PEI), respectivel y. Enzyme activities of those films on specific substrates (glucose an d H2O2) were examined by coloring reaction of dye DA67. A multienzyme film containing GOD layer and POD layer was prepared by alternate adso rption of POD/PSS followed by PEI/GOD. Sequential redox reaction of gl ucose/H2O2/DA67 was demonstrated successfully with this supramolecular system. (C) 1996 John Wiley & Sons, Inc.