THERE ARE MULTIPLE FORMS OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE IN PROSTATE-CANCER CELLS AND NORMAL PROSTATE TISSUE

We analyzed glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expressio n in normal and malignant human prostate tissues, normal rat prostate, and Dunning R-3327 rat prostate cancer cell lines. We detected multip le forms of GAPDH in Dunning cancer cell lines by two-dimensional prot ein electrophoresis and Western analysis. Five forms of GAPDH that dif fered by isoelectric point were detected for each of the two metastati c Dunning cell lines; while four or fewer forms were detected fur Dunn ing cell lines with low metastatic ability. We also detected multiple forms of GAPDH in normal and malignant human prostate specimens by two dimensional protein electrophoresis and immunohistochemical analysis. GAPDH was undetectable in normal human prostate secretory epithelium by immunohistochemistry, but was abundant in nuclei of normal basal ce lls and stromal cells. In human-prostate cancer specimens, there was a rough correlation between cytoplasmic staining for GAPDH and tumor gr ade, but GAPDH staining was extremely heterogenous. GAPDH was abundant in nuclei of some high-grade human prostate tumors. Both of the human prostate cancer bone metastases analyzed with immunohistochemistry ha d markedly elevated cytoplasmic GAPDH expression. We conclude that mul tiple forms of GAPDH may play diverse roles in normal prostate tissue and in prostate cancer. (C) 1996 Wiley-Liss, Inc.