C EBP-BETA IS A NEGATIVE REGULATOR OF HUMAN PAPILLOMAVIRUS TYPE-11 INKERATINOCYTES/

We have evaluated the impact of the CCAAT enhancer-binding protein (C/ EBP) transcription factors on human papillomavirus type 11 (HPV11). C/ EBP beta is in nuclei of cultured foreskin keratinocytes and binds its consensus sequence in HPV11 DNA. We have used the novel approach of d epleting the availability of C/EBPs in vivo using nuclease-resistant o ligomers containing C/EBP DNA binding sites. In cultured foreskin kera tinocytes containing replicating HPV11 DNA, levels of both HPV11 trans cripts and HPV DNA increase after treatment with oligomers containing the C/EBP beta DNA binding motif. These results indicate that C/EBP be ta is a repressor for HPV11 in keratinocytes.