A GENETIC-LINKAGE MAP FOR PINUS-RADIATA BASED ON RFLP, RAPD, AND MICROSATELLITE MARKERS

A genetic linkage map for radiata pine (Pinus radiata D. Don) has been constructed using segregation data from a three-generation outbred pe digree. A total of 208 loci were analyzed including 165 restriction fr agment length polymorphism (RFLP), 41 random amplified polymorphic DNA (RAPD) and 2 microsatellite markers. The markers were assembled into 22 linkage groups of 2 or more loci and covered a total distance of 13 82 cM. Thirteen loci were unlinked to any other marker. Of the RFLP lo ci that were mapped, 93 were detected by loblolly pine (P. taeda L.) c DNA probes that had been previously mapped or evaluated in that specie s. The remaining 72 RFLP loci were detected by radiata pine probes fro m a PstI genomic DNA library. Two hundred and eighty RAPD primers were evaluated, and 41 loci which were segregating in a 1:1 ratio were map ped. Two microsatellite markers were also placed on the map. This map and the markers derived from it will have wide applicability to geneti c studies in P. radiata and other pine species.