PREGNANCY WITH SPERM ASPIRATION FROM THE HEAD OF THE EPIDIDYMIS IN VAS-DEFERENS AGENESIS

From a male affected of agenesis of corpus and cauda epididymis and va s deferens, sperms were surgically obtained aspirating epididymal cont ent with the help of a surgical microscope. Motile sperms were separat ed after discontinuous Percoll gradient centrifugation and used to ins eminate in vitro the spouse's oocytes 8 hrs. after aspiration accordin g to the current techniques. Twenty hours later, oocytes were checked for fertilization and transferred to trash culture medium. From 13 pre ovulatory oocytes only one was fertilized and transferred to the Fallo pian tube at the 6 cells stage. Fourteen days after embryo transfer, s erum HCG concentration was 320 mU/ml and on day 29th a 5mm fetal sac s howing heart beats was detected by transvaginal sonography. After an u neventful 38 weeks gestation a normal baby girl weighing 2 800 g was d elivered.