INCREASED PRODUCTION OF BETA-CYCLODEXTRIN USING AN AQUEOUS 2-PHASE SYSTEM

beta-Cyclodextrin(beta-CD) was produced by cyclodextrin glycosyltransf erase(CGTase) in aqueous two-phase system. beta-CD production from sol uble starch was catalyzed by CGTase in dextran-rich bottom phase, and the beta-CD produced was transferred to PEG-rich top phase in aqueous two-phase system, composed of 7% (w/w) polyethylene glycol(M(r) 20,000 ) and 10%(w/w) dextran(M(r) 38,900). Partition coefficients of beta-CD and CGTase were 1.5 and 0.25, respectively. The total productivity of beta-CD in aqueous two-phase system was about 3 times of that in dext ran phase.