CONSTITUTIVE EXPRESSION OF NITRATE REDUCTASE CHANGES THE REGULATION OF NITRATE AND NITRITE TRANSPORTERS IN CHLAMYDOMONAS-REINHARDTII

The effect of constitutive expression of nitrate reductase (NR) on the regulation pattern of nitrite and nitrate transporters (NT) has been analysed in Chlamydomonas reinhardtii. Strain Tx11-8, bearing the chim eric Nia1 gene for NR under the control of the cabll-1 gene promoter, showed a constitutive NR which was inactive in ammonium-grown cells. I n contrast to the wild-type, incubation of Tx11-8 in ammonium media co ntaining nitrate at micromolar concentrations resulted in reactivation of inactive NR and excretion of nitrite. In addition, negligible amou nts of NT gene transcripts were induced by nitrate in this strain. How ever, NT transcripts from Tx11-8 cells were induced in amounts similar to those in the wild-type, by preventing constitutive NR expression f rom the cabll-1 gene promoter in the dark. Strains lacking high-affini ty NT and nitrite reductase activity and having the chimeric Nia1 gene have been constructed by genetic crosses. All these strains were sens itive to chlorate in ammonium containing media and excreted nitrite wh en nitrate was present at millimolar concentrations. Spontaneous chlor ate-resistant mutants isolated from these strains lacked constitutive NR activity though they constitutively expressed NR mRNA. The data str ongly suggest that nitrate plays an important post-transcriptional reg ulatory role and that NR is involved in the regulation of the nitrate/ nitrite transport activities in C. reinhardtii.