CDNA CLONING OF A MOUSE PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE RECEPTOR

A cDNA clone for mouse pituitary adenylate cyclase-activating polypept ide (PACAP) receptor (PACAP-R) was obtained from the brain using rever se transcription-polymerase chain reaction (RT-PCR). The recombinant P ACAP receptor expressed in COS cells bound PACAP with about 1000-times higher affinity than vasoactive intestinal polypeptide (VIP), and PAC AP stimulated adenylate cyclase through the cloned PACAP receptor. The mouse PACAP receptor consists of 496 amino acids, contains seven tran smembrane segments and has 98.4%, 93.0%, and 92.5% identity with the r at, bovine, and human PACAP-R, respectively.