EXOGENOUS HEPATOCYTE GROWTH-FACTOR MARKEDLY STIMULATES LIVER-REGENERATION FOLLOWING PORTAL BRANCH LIGATION IN DOGS

Portal branch ligation (PBL) or embolization prior to extensive hepate ctomy has been employed to increase the functional reserve of the rema ining liver. This study investigated the effects of human recombinant hepatocyte growth factor (rh-HGF) on liver regeneration following PBL in dogs. Beagle dogs were subjected to PBL and were divided into two g roups, a control group (n = 11) without rh-HGF and a treated group (n = 12) receiving postoperative rh-HGF at 250 ng/kg via the portal vein. Dogs were killed 72 h or 14 days following PBL. We studied the change s in serum HGF level, DNA synthesis of the liver, heptocyte size, live r weight, and liver function tests. In the HGF group, the ratio of who le liver weight to body weight increased significantly, and both ligat ed and nonligated lobes showed marked increases in weight. The nonliga ted lobes in the HGF group showed significant increases in both DNA sy nthesis and hepatocyte size. Moreover, ligated lobes in the HGF group showed an increase in DNA synthesis without hypertrophy compared with the control group. Administration of rh-HGF did not significantly affe ct liver function tests. Ligation of the portal branch supplying the p ortion of liver to be resected, coupled with the administration of rh- HGF, is a useful strategy to increase hepatic reserve in advance of ma jor hepatectomy.