STUDY OF LYMPHOCYTE SUBPOPULATIONS IN PERIPHERAL-BLOOD AND SECONDARY LYMPHOID ORGANS IN THE GOAT USING MONOCLONAL-ANTIBODIES TO SURFACE-MARKERS OF BOVINE LYMPHOCYTES

Authors
NAVARRO JA CARO MR SEVA J ROSILLO MC GOMEZ MA GALLEGO MC
Citation
Ja. Navarro et al., STUDY OF LYMPHOCYTE SUBPOPULATIONS IN PERIPHERAL-BLOOD AND SECONDARY LYMPHOID ORGANS IN THE GOAT USING MONOCLONAL-ANTIBODIES TO SURFACE-MARKERS OF BOVINE LYMPHOCYTES, Veterinary immunology and immunopathology, 51(1-2), 1996, pp. 147-156
Citations number
19
Categorie Soggetti
Immunology,"Veterinary Sciences
Journal title
Veterinary immunology and immunopathology → ACNP
ISSN journal
01652427
Volume
51
Issue
1-2
Year of publication
1996
Pages
147 - 156
Database
ISI
SICI code
0165-2427(1996)51:1-2<147:SOLSIP>2.0.ZU;2-#
Abstract
Monoclonal antibodies (mAbs) to surface markers of bovine lymphocytes MHC I, MHC II, B-cells, T-cells (CD2, CD4, CD8 and gamma/delta) and in terleukin-2 (IL-2) receptor were tested in the goat by flow cytometry and using immunohistochemical methods. Samples from peripheral blood a nd secondary lymphoid organs (mesenteric lymph nodes, spleen and ileal Peyer's patch) were studied. The percentage of positive cells obtaine d by flow cytometry and its compartmentalisation in different tissue s ections showed that the mAbs against MHC I, MHC II, CD2, CD4, CD8, gam ma/delta and IL-2 receptor recognised lymphocyte subpopulations simila r to those present in the bovine. However, the mAbs tested on B-cells reacted only partially in the recognition of this subpopulation.