MULTIPLE REGULATION OF CARP (CYPRINUS-CARPIO L) MACROPHAGES AND NEUTROPHILIC GRANULOCYTES BY SERUM FACTORS - INFLUENCE OF INFECTION WITH ATYPICAL AEROMONAS-SALMONICIDA
Bml. Verburgvankemenade et al., MULTIPLE REGULATION OF CARP (CYPRINUS-CARPIO L) MACROPHAGES AND NEUTROPHILIC GRANULOCYTES BY SERUM FACTORS - INFLUENCE OF INFECTION WITH ATYPICAL AEROMONAS-SALMONICIDA, Veterinary immunology and immunopathology, 51(1-2), 1996, pp. 189-200
Normal carp serum contains inhibitory and stimulatory factors for macr
ophage and neutrophilic granulocyte respiratory burst activity. As sti
mulatory factors were only effective in combination with phorbol myris
tate actetate (PMA) activation, it is concluded that they are probably
linked to protein kinase C activation. Both the stimulatory and inhib
itory factors are heat stable. Macrophage- and neutrophilic granulocyt
e-enriched cell fractions from the pronephros of carp had high respira
tory burst- and high bactericidal in vitro responses to virulent atypi
cal Aeromonas salmonicida bacteria. Serum factors were inhibitory for
the A, salmonicida induced respiratory burst activity. No change in in
hibitory or stimulatory serum factors could be observed during a 12-da
y challenge experiment with A. salmonicida, or during a rechallenge of
survivors from a previous sub-lethal infection. The sensitivity of ma
crophages and neutrophilic granulocytes to stimulation of respiratory
burst activity by PMA was not significantly altered. Culture supernata
nts from PHA pre-treated lymphocytes stimulated the respiratory burst
activity of macrophages and neutrophilic granulocytes suggesting that
serum factors may partially be lymphocyte derived. Carp macrophages an
d neutrophilic granulocytes undergo a multiple regulation by stimulato
ry and inhibitory factors that will probably prevent the cells from ca
using tissue damage during latent periods, but which enables rapid rea
ction when fast induction of activity is required.