HETEROGENEITY IN RADIOSENSITIVITY OF HUMAN-DIPLOID FIBROBLASTS FROM KELOIDS AND NORMAL SKINS

Colony-forming ability was employed in evaluating the susceptibility t o in vitro gamma ionizing radiation in human diploid skin fibroblasts (HDF). Twelve pairs of HDF, each composed of fibroblasts from excised keloid lesion and local normal skill tissue as its control, were studi ed in patients with clinically persistent keloids. Parameters of radio sensitivity, both D-0 and D-10, and growth kinetics were examined. The radiosensitivity in three of the 12 keloids (25%) were demonstrated s ignificantly increased than their counterpart controls, even though no difference in growth kinetics in between. Moreover, a broad range in the radiosensitivity of fibroblast cells was demonstrated and it is su ggested that there is a great heterogeneity of cellular response to ra diation in HDF. (C) 1996 Academic Press Limited