DETECTION OF PROTEIN-PROTEIN INTERACTIONS IN THE NERVOUS-SYSTEM USINGTHE 2-HYBRID SYSTEM

Citation
A. Nishimune et al., DETECTION OF PROTEIN-PROTEIN INTERACTIONS IN THE NERVOUS-SYSTEM USINGTHE 2-HYBRID SYSTEM, Trends in neurosciences, 19(7), 1996, pp. 261-266
Citations number
45
Categorie Soggetti
Neurosciences,Neurosciences
Journal title
ISSN journal
01662236
Volume
19
Issue
7
Year of publication
1996
Pages
261 - 266
Database
ISI
SICI code
0166-2236(1996)19:7<261:DOPIIT>2.0.ZU;2-L
Abstract
Specific interactions between proteins regulate nearly all cellular pr ocesses. In the nervous system specialized processes such as neuronal proliferation, differentiation and targeting, synapse formation and ne urotransmitter release are all tightly controlled by cascades of prote in-protein interactions. The extent and nature of these interactions i s therefore a question of fundamental importance. The two-hybrid syste m, which is beginning to be widely applied in many other areas of cell biology, offers a novel and sensitive technique for the identificatio n and analysis of these protein-protein interactions.