A. Nishimune et al., DETECTION OF PROTEIN-PROTEIN INTERACTIONS IN THE NERVOUS-SYSTEM USINGTHE 2-HYBRID SYSTEM, Trends in neurosciences, 19(7), 1996, pp. 261-266
Specific interactions between proteins regulate nearly all cellular pr
ocesses. In the nervous system specialized processes such as neuronal
proliferation, differentiation and targeting, synapse formation and ne
urotransmitter release are all tightly controlled by cascades of prote
in-protein interactions. The extent and nature of these interactions i
s therefore a question of fundamental importance. The two-hybrid syste
m, which is beginning to be widely applied in many other areas of cell
biology, offers a novel and sensitive technique for the identificatio
n and analysis of these protein-protein interactions.