ELECTROCHEMICAL FIXATION TECHNIQUES .2. ELECTROCHEMICAL DOG-BODY FIXATION - HISTOLOGICAL STUDY

This is the first attempt to harden all organs of a body together with out excising them. This process was accomplished in bottom-belted, gas trointestinal (GI) or intravenously (IV) catheterized dog cadavers so as to influx an electrolytic solution containing formaldehyde (ESF), T he IV influx of ESF was found to be the best perfusion pathway, After 48 h of immersion in ESF, 24 h current time of 17.5 A of current inten sity, 24 degrees to 56 degrees C, we ended up with thoroughly fixed do g cadavers that were wrapped with ethyl alcohol:glycerol gauzes and st ored in electrochemical whole body fixation is described, plastic bags at room temperature, Optical microscopy of every sliced tissue showed normal blood vessels, neurons, glial and Purkinje cells and their nuc lei of brain and cerebellum, respectively, Cardiac muscle fibers were of normal appearance, Kidney Bowman's capsule and space were found to be normal except for vacuolarly degenerated tubules, Small intestine s howed normal epithelial cells and crypts of Lieberkuhn. In liver, sinu soids were normally arrayed but showed vacuolar cell degeneration, Her ein a method to attain an electrochemical whole body fixation is descr ibed.