BIOTIN AND FLUORESCEIN LABELING OF BIOMOLECULES BY ACTIVE ESTERS OF 1-PHENYLPYRAZOLIN-5-ONES

Authors
KREMSKY JN PLUSKAL M CASEY S PERRYOKEEFE H KATES SA SINHA ND
Citation
Jn. Kremsky et al., BIOTIN AND FLUORESCEIN LABELING OF BIOMOLECULES BY ACTIVE ESTERS OF 1-PHENYLPYRAZOLIN-5-ONES, Tetrahedron letters, 37(25), 1996, pp. 4313-4316
Citations number
13
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
37
Issue
25
Year of publication
1996
Pages
4313 - 4316
Database
ISI
SICI code
0040-4039(1996)37:25<4313:BAFLOB>2.0.ZU;2-3
Abstract
The synthesis of 1-phenylpyrazolin-5-one carboxylate esters of biotin and fluorescein and their specific reactivity with amines under mild c onditions to label various synthetic biopolymers such as oligonucleoti des, peptides and PNA's is described. Copyright (C) 1996 Elsevier Scie nce Ltd.