This paper describes the mechanism generating the beautiful wing color
s of various male butterflies and the relationship between the wing ma
terial and the color appearance. The microstructure of the scales cove
ring the upper surface of the wings was analyzed with the aid of a sca
nning electron microscope. The basic mechanism of color generation of
structurally colored scales is determined for the first time in accord
ance with the theory of optical interference in thin film layers using
a model of wing scales. Optical properties were found in relation to
the three-dimensional spectral reflectance of the samples, and differe
nces were observed between the brightness perceived in subjective eval
uations and calculated values based on the reflective spectra of the s
tructurally colored wings. The results of this study suggest that the
microroughness of the upper wing surface may influence the perceived g
loss of structurally colored wings.