IDENTIFICATION OF AN AMINO-ACID SIGNATURE SEQUENCE PREDICTIVE OF PROTEIN G-INHIBITABLE IGG(3)-BINDING ACTIVITY IN GROUP-A STREPTOCOCCAL IGG-BINDING PROTEINS
Td. Pack et al., IDENTIFICATION OF AN AMINO-ACID SIGNATURE SEQUENCE PREDICTIVE OF PROTEIN G-INHIBITABLE IGG(3)-BINDING ACTIVITY IN GROUP-A STREPTOCOCCAL IGG-BINDING PROTEINS, Gene, 171(1), 1996, pp. 65-70
Sequence comparison of six known group-A streptococcal IgG-binding pro
teins, sharing the common property of protein G-inhibitable IgG(3)-bin
ding-activity, identified a highly conserved 35-amino-acid (aa) sequen
ce (74-100% similarity) within an EQ-rich central conserved core regio
n of each protein. A search of aa sequence databases identified four a
dditional proteins with > 50% similarity to this consensus sequence. A
ll of these proteins demonstrated protein G-inhibitable IgG(3)-binding
activity. Taken together, these results identify a signature sequence
that predicts the presence of a protein G-inhibitable IgG(3)-binding
domain(s) in group-A streptococcal IgG-binding proteins.