CLONING OF THE GENE ENCODING THE MITOCHONDRIAL ADENINE-NUCLEOTIDE CARRIER OF SCHIZOSACCHAROMYCES-POMBE BY FUNCTIONAL COMPLEMENTATION IN SACCHAROMYCES-CEREVISIAE

We describe the isolation and sequencing of both cDNA and genomic clon es encoding the mitochondrial ADP/ATP carrier (Anc) of Schizosaccharom yces pombe (Sp). The cDNA clone was isolated from a cDNA library of th is fission yeast by complementation of a Saccharomyces cerevisiae (Sc) strain defective in adenine nucleotide carrier. The predicted amino a cid (aa) sequence (322 aa) shared similarity with the known Anc sequen ces. It is more closely related to Neurospora crassa (Nc) Anc than to ScAnc1, 2, or 3 or Kluyveromyces lactis (Kl) Anc. Hybridization experi ments with ordered libraries of Sp genomic DNA led to the physical map ping (chromosome II, NotI-B region) and the isolation of the Sp ANC1 g ene. We also conclude that a single-copy gene encodes the Sp Anc.