The gene organization of a 7.4-kb region of the Streptomyces virginiae
(Sv) chromosome was determined. The predicted open reading frames (OR
Fs) and their predicted products, in sequence order, were (i) ada, enc
oding adenosine deaminase [EC 3.5.4.4], (ii) aat, encoding a protein h
omologous to aspartate aminotransferase [EC 2.6.1.1], (iii) secE, enco
ding a protein involved in protein secretion, (iv) vbr A, encoding a N
usG-like protein involved in antitermination of transcription as descr
ibed by Okamoto et al. [J. Biol. Chem. 267 (1992) 1093-1098], and (v)
rplKAJL, encoding the large subunits of the ribosomal proteins L11, L1
, L10 and L12. Six of the ORFs (secE-rplL) were oriented in the same d
irection, but the other two (ada and aat) had the opposite orientation
. The gene organization of the secE-rplL region in Sv was identical to
that in Escherichia coli.