ORGANIZATION IN THE ADA-RPLL REGION OF STREPTOMYCES-VIRGINIAE

The gene organization of a 7.4-kb region of the Streptomyces virginiae (Sv) chromosome was determined. The predicted open reading frames (OR Fs) and their predicted products, in sequence order, were (i) ada, enc oding adenosine deaminase [EC 3.5.4.4], (ii) aat, encoding a protein h omologous to aspartate aminotransferase [EC 2.6.1.1], (iii) secE, enco ding a protein involved in protein secretion, (iv) vbr A, encoding a N usG-like protein involved in antitermination of transcription as descr ibed by Okamoto et al. [J. Biol. Chem. 267 (1992) 1093-1098], and (v) rplKAJL, encoding the large subunits of the ribosomal proteins L11, L1 , L10 and L12. Six of the ORFs (secE-rplL) were oriented in the same d irection, but the other two (ada and aat) had the opposite orientation . The gene organization of the secE-rplL region in Sv was identical to that in Escherichia coli.