Pretreatment of Estonian oil shale by thermophilic Sulfolobus acidocal
darius and mesophilic Thiobacillus ferrooxidans in order to remove ino
rganic sulphur compounds (pyrite) has been studied. Shake flask experi
ments showed bacterial growth on the internal pyrite of the shale and
no substrate toxicity. The bioreactor experiments demonstrated that th
e oxidation of pyrite by S. acidocaldarius is especially fast (96 % in
four days). However, to evaluate the economic feasibility of the proc
ess, the high acid consumption of this oil shale has to be taken into
consideration.