PLASMA-VOLUME DETERMINATION WITH THE DYE ICG IN INTRAVASCULAR VOLUME SHIFT - REPEATED MEASUREMENTS WITH THE DYE-DILUTION METHOD BEFORE AND AFTER HEMORRHAGE AND AFTER RETRANSFUSION OF AUTOLOGOUS BLOOD IN MAN

Objective: The determination of the current intravascular plasma volum e with the dye dilution method is of major interest in therapeutic int ervention and scientific research. The use of the rapidly eliminated d ye indocyanine green (ICG) allows to perform plasma volume measurement s serially and to observe a time course. The following questions shoul d be investigated in the present study: 1. Do the plasma volume (PV) v alues measured by the ICG method in this study agree with data of othe r authors with special respect to basic values for further clinical in vestigations, and 2. Do the measured differences coincide with the wit hdrawn and retransfuse plasma volume? Design: Prospective study (on ea ch 20 healthy female and male volunteers). Setting: Research laborator y of a university department of anesthesiology. Participants: Each 20 healthy female and male volunteers. Interventions: Measurement of PV w ith ICG both before and after withdrawal of 10% of blood volume and af ter retransfusion. Results: The plasma volume per body surface area (P V/BSA) was in the group of female volunteers 1639 +/- 198 and in male volunteers 1637 +/- 224 ml/m(2) (mean +/- SD). The withdrawn amount of plasma volume (EPV) was 188+/-23 in the female group and 149 +/- 26 m l/m(2) in the male group. Determined by the ICG method the difference for the withdrawal was in female volunteers 198 +/- 174 and in male vo lunteers 171 +/- 158 ml/m(2) and for the retransfusion in the female g roup 190 +/- 169 and in the male group 142 +/- 154 ml/m(2). Conclusion s: The mean differences in plasma volume measured by the ICG method bo th before and after withdrawal and after retransfusion of autologous b lood were in good agreement with the withdrawn amount of plasma volume . The large standard deviation has to be considered in clinical assess ment of an individual measurement.