OBTAINING THE CONCENTRATION-DEPENDENT DIFFUSION-COEFFICIENT FROM ULTRAFILTRATION EXPERIMENTS - APPLICATION TO HYALURONATE

Citation
Si. Barry et al., OBTAINING THE CONCENTRATION-DEPENDENT DIFFUSION-COEFFICIENT FROM ULTRAFILTRATION EXPERIMENTS - APPLICATION TO HYALURONATE, Biopolymers, 39(1), 1996, pp. 1-11
Citations number
28
Categorie Soggetti
Biology
Journal title
ISSN journal
00063525
Volume
39
Issue
1
Year of publication
1996
Pages
1 - 11
Database
ISI
SICI code
0006-3525(1996)39:1<1:OTCDFU>2.0.ZU;2-D
Abstract
A method for determining the concentration-dependent mutual diffusion coefficient D(C) of a macromolecule-solvent combination over a wide ma cromolecular concentration range is presented. All necessary data are gathered from a single experiment, in which polymer concentration prof iles are measured during one-dimensional dead-end ultrafiltration. Bas ed on these profiles, the convection-diffusion equation is used to ded uce the dependence of D on C. To demonstrate the utility of the approa ch, studies using sodium hyaluronate dissolved in either 10 mM NaCl or a phosphate buffer were carried out. For hyaluronate (HA) in 10 mM Na Cl, the mutual diffusion coefficient varies approximately linearly wit h concentration according to D(C) = 4.1 X 10(-6) C-0.96 cm(2)/s in the range 0 less than or equal to C less than or equal to 0.6 mass %, for C expressed in mass %. However, transition points (slope changes) in the D(C) curve are present at C approximate to 0.7 mass % and C approx imate to 1.4 mass %. For HA in the phosphate buffer, the mutual diffus ion coefficient is well described by D(C) = 1.9 X 10(-6) C-0.825, for 0 less than or equal to C less than or equal to 1.8 mass %. These valu es agree well with previously published data. The technique is robust, and permits reasonably high polymer concentrations to be easily studi ed. (C) 1996 John Wiley & Sons, Inc.