Ra. Anderson et al., DIETARY CHROMIUM EFFECTS ON TISSUE CHROMIUM CONCENTRATIONS AND CHROMIUM ABSORPTION IN RATS, The Journal of trace elements in experimental medicine, 9(1), 1996, pp. 11-25
Chromium (Cr) absorption is low (<1%) and there is a need to find Cr c
ompounds that are absorbed better than inorganic Cr salts. Therefore,
the incorporation of nine different chromium (Cr) compounds on tissue
Cr concentration of 6-week male Wistar rats was investigated. Chromium
compounds tested were Cr chloride (Cr chloride), Cr acetate (Cr aceta
te), Cr potassium sulfate (CrAlum), Cr trihistidine (Cr histidine), Cr
triglycine (Cr glycine), Cr trinicotinic acid (CrNA), Cr dinicotinic
acid dihistidine (CrNA-HIS), Cr tripicolinic acid (Cr picolinate), and
Cr dinicotinic acid diglycine cysteine glutamic acid (CrNA-AA). Compl
exes were fed to weanling rats for 3 weeks at 5,000 ng of Cr/g of diet
. Basal control diet was a cornstarch-based diet containing 30 ng Cr/g
. Chromium incorporation into the kidney was greatest for CrNA-AA comp
lex (850 ng/g dry wt) followed by CrAlum (407 ng/g), Cr acetate (397),
CrNA-HIS (394), Cr picolinate (368), Cr glycine (343), Cr nicotinate
(166), Cr chloride (74), CrHIS (49), and control (23 ng/g). Chromium c
oncentration of the liver was greatest for the Cr picolinate compound
(50 ng/g) followed by CrNA-AA and Cr acetate. Liver Cr concentrations
of remaining complexes were not significantly different from those of
the control animals that received no added Cr. Chromium concentrations
were significantly greater in the kidney than those for the liver, sp
leen, heart, lungs, and gastrocnemius muscle. Supplemental Cr did not
affect tissue zinc arid copper but did alter tissue iron concentration
s. Absorption of radioactive forms of Cr did not explain the differenc
es in tissue Cr concentrations. Chromium absorption after 4 hours and
retention after 24 hours were not significantly different for the form
s of Cr tested. These data demonstrate that Cr concentrations are grea
t est in the kidney and that the form of dietary Cr significantly affe
cts tissue Cr concentrations. Absorption of Cr does not correlate with
tissue Cr concentrations and blood Cr is not in equilibrium with tiss
ue Cr stores. (C) 1996 Wiley-Liss, Inc.