SEROMONITORING OF ANTIBODIES IN DOGS BY INDIRECT ELISA FOLLOWING POST-BITE RABIES VACCINATION

An indirect ELISA was standardized with human diploid cell vaccine who le virus as the antigen and seromonitoring of 149 dogs which had recei ved post-bite vaccination for rabies was carried out. Serological resp onse against two cell culture vaccines and one Semple's vaccine was as sayed. The optical density (OD) values recorded for the 3 vaccines fel l more or less in the same range. However, the cell culture vaccines i ndicated significantly high OD values statistically. No post-vaccinati on reaction was observed in any of the vaccinated dogs either with Sem ple's or cell culture vaccine. The study revealed the utility of ELISA as a tool for seromonitoring of rabies antibodies and nerve tissue ra bies vaccine as an economic alternative where post-bite cell culture r abies vaccination is not practicable.