DYNAMIN-II BINDS TO THE TRANS-GOLGI NETWORK

Two dynamin II - specific antibodies were used to detect dynamin II at tached to highly purified Golgi membranes of HepG2 cells. Within the G olgi apparatus dynamin II is predominantly localized to the trans-Golg i network, as shown by immunoelectron microscopy. Increased binding of dynamin II to the trans-Golgi network in the presence of GTP-gamma-S and a reduced binding upon stimulation of secretion by phorbolester in dicates that dynamin II may have a function in post-Golgi vesicle tran sport. (C) 1996 Academic Press, Inc.