PROCESSING OF ANTIBODIES BOUND TO B-CELL LYMPHOMAS AND OTHER HEMATOLOGICAL MALIGNANCIES

In an attempt to recognize general patterns in the processing of Abs ( antibodies) bound to the surface of tumor cells, eight monoclonal anti bodies were tested on 10 hematological malignancies of various histolo gical types. The results were compared with previous findings obtained with carcinomas, melanomas, and gliomas, using some of the same antib odies. The data demonstrated that some B-cell lymphomas appear to be u nusual in that Abs were unable to bind to them irreversibly; except fo r those Abs that were rapidly internalized, none of the Abs tested was able to bind irreversibly to the B-cell lymphomas Raji or RL. In cont rast, most Abs bound irreversibly to the tumors of other histological types, including other hematological tumors. Irreversible Ab binding t o B-cell lymphomas was achieved by cross-linking the Abs on the cell s urface. Such differences between cell lines may be due to differences in the supramolecular structure of the surface membrane, which affect the frequency or stability of bivalent Ab binding. The Ab binding inte raction could not be described in terms of ''functional affinity.'' Th ese results may lead to improvements in the use of Abs for tumor immun otherapy and for other purposes.