SIMPLIFIED MEASUREMENT OF PROTEIN-BOUND IODINE WITH EPITHERMAL NEUTRON-ACTIVATION ANALYSIS

A refined method for the measurement of protein-bound iodine (PBI) in blood serum was demonstrated by the use of epithermal neutron activati on analysis (ENAA). PBI in ammonium sulfate-precipitated serum protein , after epithermal neutron activation, was determined by high resoluti on gamma-ray spectrometry. From our results, the PBI concentration was 67.8 +/- 2.2 ng/mL. Good agreement was obtained with published data, ranging from 40 to 80 ng/mL, which had been obtained using different a nalytical techniques. The validity of these techniques for PBI has bee n born out by a very good accuracy and simplicity without temperature dependence.