CHLORAMINE-T INDUCED BINDING OF MONOCLONAL-ANTIBODY B72.3 TO CONCANAVALIN-A

Authors
COLE WC JHINGRAN SG
Citation
Wc. Cole et Sg. Jhingran, CHLORAMINE-T INDUCED BINDING OF MONOCLONAL-ANTIBODY B72.3 TO CONCANAVALIN-A, Nuclear medicine and biology, 20(5), 1993, pp. 649-655
Citations number
44
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging
Journal title
Nuclear medicine and biology
ISSN journal
09698051 → ACNP
Volume
20
Issue
5
Year of publication
1993
Pages
649 - 655
Database
ISI
SICI code
0969-8051(1993)20:5<649:CIBOMB>2.0.ZU;2-A
Abstract
The effects of chloramine-T (CT) on monoclonal antibody B72.3 were stu died with particular reference to Con-A lectin binding. After exposure to chloramine-T concentrations from 0.8 to 4.0 mg/mL (115-574 mol CT/ mol B72.3), B.72.3 showed progressive binding to agarose-linked Con-A. This behavior was paralleled by decreasing immunoreactivity and incre asing fragmentation and aggregation of B72.3 demonstrated by SDS PAGE and size exclusion HPLC.