REGULATION AND LOCALIZATION OF CELLULAR RETINOL-BINDING PROTEIN, RETINOL-BINDING PROTEIN, CELLULAR RETINOIC ACID-BINDING PROTEIN (CRABP), AND CRABP-II IN THE UTERUS OF THE PSEUDOPREGNANT RAT
Ra. Bucco et al., REGULATION AND LOCALIZATION OF CELLULAR RETINOL-BINDING PROTEIN, RETINOL-BINDING PROTEIN, CELLULAR RETINOIC ACID-BINDING PROTEIN (CRABP), AND CRABP-II IN THE UTERUS OF THE PSEUDOPREGNANT RAT, Endocrinology, 137(7), 1996, pp. 3111-3122
Three members of the superfamily of small intracellular carrier protei
ns for Lipophilic compounds are cellular retinol-binding protein (CRBP
), cellular retinoic acid-binding protein (CRABP), and cellular retino
ic acid-binding protein II (CRABP II). Retinol-binding protein (RBP) i
s a secreted protein that binds and solubilizes vitamin A for transpor
t. Here we report the coordinate regulation of RBP, CRBP, retinol, and
CRABP II in the uterus of the pseudopregnant rat. In the proliferativ
e stage of the uterus, which was induced by PMSG, the messenger RNA (m
RNA) and protein levels of RBP and CRBP as well as retinol levels sign
ificantly decreased. This pattern of regulation was duplicated by estr
ogen treatment of prepubertal rats. In addition, CRBP and RBP were fou
nd to be colocalized to the stromal cells of the rat uterus by immunoh
istochemistry and [S-35]methionine-labeled affinity chromatography, re
spectively, and were not detected in other cell populations. CRABP II
mRNA and protein expression were up-regulated in the proliferative pha
se of the uterus brought about by PMSG injection or, alternatively, by
estrogen treatment of prepubertal rats. CRABP II was localized to the
surface epithelium, but was not seen elsewhere, including glandular e
pithelium. Immunolocalization of CRABP showed staining of the smooth m
uscle and stromal cells of the uterus. The appearance of CRABP in the
stroma of the uterus also correlated with PMSG injection as well as es
trogen treatment. Although estrogen induced the appearance of both bin
ding proteins, CRABP mRNA levels peaked between 4-24 h postestrogen tr
eatment, whereas CRABP II mRNA levels continued to rise 48 h postestro
gen treatment. These data demonstrate an important role for vitamin A
and retinoid-binding proteins in rat uterine physiology.