Kd. Gillis et al., PROTEIN-KINASE-C ENHANCES EXOCYTOSIS FROM CHROMAFFIN CELLS BY INCREASING THE SIZE OF THE READILY RELEASABLE POOL OF SECRETORY GRANULES, Neuron, 16(6), 1996, pp. 1209-1220
We have used membrane capacitance measurements to assay Ca2+-triggered
exocytosis in single bovine adrenal chromaffin cells. Brief applicati
on of phorbol ester (PMA) enhances depolarization-evoked exocytosis se
veralfold while actually decreasing the Ca2+ current. Ca2+ metabolism
is unchanged. Three different protocols were used to show that PMA inc
reases the size of the readily releasable pool of secretory granules.
PMA treatment leads to a large increase in amplitude, but little chang
e in the time course of the exocytic burst that results from rapid ele
vation of [Ca2+](i) upon photolysis of DM-Nitrophen. Thus, PKC appears
to affect a late step in secretion but not the Ca2+ sensitivity of th
e final step.