QUANTITATIVE-DETERMINATION OF TRITERPENES IN EXTRACTS AND PHYTOPREPARATIONS OF CENTELLA-ASIATICA (L) URBAN

A new method for separation and quantitative determination of triterpe nes in Centella asiatica herb and its pharmaceutical preparations has been developed. Separation of all pharmacologically relevant triterpen es (asiaticoside, madecassoside, asiatic- and madecassic acid) was ach ieved by HPLC employing the reversed-phase separation system acetonitr ile/water on RP 18 columns at a detection wavelength of 205 nm. Quanti fication was carried out by the method of external standard with calib ration curves for all triterpenes having correlation coefficients very near to 1. The method is suitable for fingerprint analysis and standa rdization of all Centella asiatica preparations.