CHARACTERIZATION OF DYESTUFF-CONTAINING LIPOSOME BY CAPILLARY ZONE ELECTROPHORESIS USING ONLINE CHEMILUMINESCENCE DETECTION

Eosin Y-containing liposome solution as a sample was migrated and dete cted by capillary zone electrophoresis for the first time with a chemi luminescence detection using peroxyoxalate system. Two peaks were reco rded on the electropherogram; the first one was due to Eosin Y entrapp ed in liposome and the second one due to free Eosin Y in the bulk solu tion. The electropherogram comprising the two peaks offered various in formations of liposome, such as size distribution, stability, permeabi lity, or surface charge.