DIRECT OBSERVATION OF SOLUTES TRAPPING ON A SURFACE-INERT REVERSED-PHASE COLUMN

The solute trapping process onto the surface-inert reversed phase pack ing was directly investigated with chromato-videoscope. The Fluorescen ce intensity of separating dansyl (DNS) amino acids in the column was detected by video camera and densitogarm of solutes along the column l ength was obtained with computer-processing of the video images. Band widths of trapped DNS amino acids were narrow in the absence of BSA in sample solution. However, in the presence of BSA, band shapes turned broad according to increase of the concentration of BSA or injected vo lume of the sample.