A PANEL OF VNTR MARKERS IN PIGS

By cloning tandemly repeated sequences from the pig genome by use of n on-porcine minisatellite probes for library screening, five novel poly morphic VNTR loci were isolated: three minisatellites and two satellit e-like loci. Four of them could be mapped onto chromosomes by Linkage analysis and/or in situ hybridization. They were assigned to Chromosom es (Chrs) 5, 6, 14, and 16. Physical mapping on both presumed satellit es and on one of the minisatellites revealed that the former resided n ear or at the centromere and the latter towards the chromosome ends. T he location of the minisatellite is of particular interest since, toge ther with data on three other minisatellites previously isolated, it s upports the idea that, as in humans, minisatellites may preferentially be subtelomeric also in pigs.