PORCINE LINKAGE AND CYTOGENETIC MAPS INTEGRATED BY REGIONAL MAPPING OF 100 MICROSATELLITES ON SOMATIC-CELL HYBRID PANEL

Recently two main genetic maps [Rohrer et al. Genetics 136, 231 (1994) ; Archibald et al. Mamm. Genome 6, 157 (1995)] and a cytogenetic map [ Yerle et al. Mamm. Genome 6, 175 (1995)] for the porcine genome were r eported. As only a very few microsatellites are located on the cytogen etic map, it appears to be important to increase the relationships bet ween the genetic and cytogenetic maps. This document describes the reg ional mapping of 100 genetic markers with a somatic cell hybrid panel. Among the markers, 91 correspond to new localizations. Our study enab led the localization of 14 new markers found on both maps, of 54 found on the USDA map, and of 23 found on the PiGMaP map. Now 21% and 43% o f the markers on the USDA and PiGMaP linkage maps respectively are phy sically mapped. This new cytogenetic information was then integrated w ithin the framework of each genetic map. The cytogenetic orientation o f the USDA linkage maps for Chromosomes (Chrs) 3, 8, 9, and 16 and of PiGMaP for Chr 8 was determined. USDA and PiGMaP linkage maps are now oriented for all chromosomes, except for Chrs 17 and 18. Moreover, the linkage group ''R'' from the USDA Linkage map was assigned to Chr 6.