Da. Patton et al., COMPLEMENTATION OF AN ARABIDOPSIS-THALIANA BIOTIN AUXOTROPH WITH AN ESCHERICHIA-COLI BIOTIN BIOSYNTHETIC GENE, MGG. Molecular & general genetics, 251(3), 1996, pp. 261-266
The Arabidopsis thaliana biotin auxotroph biol was rendered prototroph
ic by transformation with a chimeric transgene containing the Escheric
hia coli bioA gene driven by a constitutive promoter. The bioA gene en
codes the biotin biosynthetic enzyme 7,8-diaminopelargonic acid aminot
ransferase. Unlike the untransformed control plants, transgenic plants
expressing the bacterial transgene synthesized biotin and grew to mat
urity without biotin-deficiency symptoms. These findings demonstrate t
hat bio1/bio1 mutant plants are defective in the gene encoding 7,8-dia
mino-pelargonic acid aminotransferase.