Yd. Chung et al., IDENTIFICATION OF OVARIAN ENHANCER-BINDING FACTORS WHICH BIND TO OVARIAN ENHANCER-1 OF THE DROSOPHILA GENES YP1 AND YP2, MGG. Molecular & general genetics, 251(3), 1996, pp. 347-351
It has been reported that three different DNA regions - the fat body e
nhancer and ovarian enhancers 1 and 2 - direct the tissue-specific exp
ression of yp1 and yp2 in Drosophila melanogaster. In the present stud
y, we identified ovarian enhancer 1-specific binding proteins. Electro
phoretic mobility shift assay revealed that these proteins are present
in the adult ovary, but not in adult testis or fat body. Southwestern
blot analysis showed that about 130 kDa and 40 kDa proteins, designat
ed OEF1 and OEF2, respectively, from ovarian nuclear or crude extracts
bind specifically to the ovarian enhancer 1. The two proteins were pa
rtially purified by streptavidin/agarose-DNA affinity chromatography,
and. their binding activity was confirmed by electrophoretic mobility
shift assay. These ovarian enhancer factors may play an important role
in the regulation of transcription of yp1 and yp2 in the ovary.