Mb. Reynders et al., STUDIES ON THE GROWTH, MODELING AND PIGMENT PRODUCTION BY THE YEAST PHAFFIA-RHODOZYMA DURING FED-BATCH CULTIVATION, Biotechnology letters, 18(6), 1996, pp. 649-654
As Phaffia rhodozyma is a Crabtree positive yeast, its cell yield and
pigment production are reduced at high sugar concentrations. A method
for maintaining low growth medium sugar concentrations is fed-batch cu
lture. Using a mass balance approach and Monod growth kinetics a model
is presented which describes the fed-batch culture of Phaffia rhodozy
ma and enables the calculation of a feed regime to obtain the maximum
yield of cells and pigment. Although developed on a glucose medium, th
e model was also applied successfully to a molasses-based medium.