OPTIMIZATION OF PCR TEMPLATE CONCENTRATIONS FOR IN-VITRO TRANSCRIPTION-TRANSLATION REACTIONS

Authors
RENSHAWGEGG L GUILTINAN MJ
Citation
L. Renshawgegg et Mj. Guiltinan, OPTIMIZATION OF PCR TEMPLATE CONCENTRATIONS FOR IN-VITRO TRANSCRIPTION-TRANSLATION REACTIONS, Biotechnology letters, 18(6), 1996, pp. 679-682
Citations number
4
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
18
Issue
6
Year of publication
1996
Pages
679 - 682
Database
ISI
SICI code
0141-5492(1996)18:6<679:OOPTCF>2.0.ZU;2-4
Abstract
In vitro transcription-translation reactions can be used to rapidly an d inexpensively synthesize small amounts of protein from cloned DNA se quences for biochemical studies. The use of PCR to amplify template se quences has many advantageous over conventional protocols. However, th e amount of template needs to be carefully adjusted for optimal produc tion of protein products.