ETHANOL-PRODUCTION BY RECOMBINANT ESCHERICHIA-COLI KO11 USING CRUDE YEAST AUTOLYSATE AS A NUTRIENT SUPPLEMENT

Crude yeast autolysate (10 g/l; 5.8 g solubles/l) supplemented with mi nerals and vitamins served as a nutrient supplement for ethanol produc tion by the recombinant bacterium, Escherichia coli KO11. Ethanol prod uction in this medium (46 +/- 1.7 g ethanol/l) was equivalent to that obtained using 5-15 g/l of purified, soluble, commercial protein hydro lysates (44-48 g ethanol/l). On site production of autolysates from sp ent yeast offers the potential for synergy between grain-based and lig nocellulose-based ethanol plants.