NEW KNOWLEDGE ABOUT THE PHA-LOCUS AND P(3HB) GRANULE-ASSOCIATED PROTEINS IN CHROMATIUM-VINOSUM

Authors
LIEBERGESELL M STEINBUCHEL A
Citation
M. Liebergesell et A. Steinbuchel, NEW KNOWLEDGE ABOUT THE PHA-LOCUS AND P(3HB) GRANULE-ASSOCIATED PROTEINS IN CHROMATIUM-VINOSUM, Biotechnology letters, 18(6), 1996, pp. 719-724
Citations number
14
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
18
Issue
6
Year of publication
1996
Pages
719 - 724
Database
ISI
SICI code
0141-5492(1996)18:6<719:NKATPA>2.0.ZU;2-E
Abstract
The isolation of poly(3-hydroxybutyric acid) granules of Chromatium vi nosum D was re-examined. Beside the PHA synthase and a 17 kDa protein, a 14 kDa protein was identified as predominant granule-associated pro tein. The M(r) as well as the N-terminal amino acid sequence exhibited identity to ORF5(Cv), which is located within the pha-locus of C. vin osum. In addition, sequence alignements revealed new information about ORF4(Cv), which is also located within the pha-locus, and about the 1 7 kDa protein, which exhibited homology to heat shock proteins recentl y detected in Escherichia coli.