CYCLODEXTRIN POLYMER ENCAPSULATED PARTICLES FOR SUPERCRITICAL-FLUID CHROMATOGRAPHY

Cyclodextrin polysiloxane encapsulated particles were prepared for pac ked capillary column supercritical fluid chromatography. Silica partic les were deactivated using polymethylhydrosiloxane through a dehydroco ndensation reaction, and then coated and crosslinked with a cyclodextr in-substituted polymethylsiloxane. Using a fluidized bed coating metho d, nonagglomerating polymer coated particles were obtained even when a high loading of cyclodextrin polymer (25% w/w) was used. The polariti es and enantiomeric selectivites of the packing materials were investi gated for a wide variety of chiral test solutes, including hydrocarbon , alcohol, ketone, ester, lactone, diol, acid and amine, using neat su percritical CO2 as mobile phase. The effects of temperature and pressu re on polarity and enantiomeric selectivity were also investigated. (C ) 1996 John Wiley & Sons, Inc.