STUDY OF THE FLUORESCENCE SYSTEM DEOXYGUANYLIC ACID-TB(III) AND THE DETERMINATION OF DEOXYGUANYLIC ACID

Authors
YANG JH GAO ZQ JIE NQ ZOU HB HAN RJ ZHAO WD HUANG XJ
Citation
Jh. Yang et al., STUDY OF THE FLUORESCENCE SYSTEM DEOXYGUANYLIC ACID-TB(III) AND THE DETERMINATION OF DEOXYGUANYLIC ACID, SPECT ACT A, 52(6), 1996, pp. 709-715
Citations number
3
Categorie Soggetti
Spectroscopy
Journal title
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY → ACNP
ISSN journal
13861425
Volume
52
Issue
6
Year of publication
1996
Pages
709 - 715
Database
ISI
SICI code
1386-1425(1996)52:6<709:SOTFSD>2.0.ZU;2-I
Abstract
It was found that deoxyguanylic acid (dGMP) can form a complex with Tb (III) at pH 6.9-7.5, which then emits the strong characteristic fluore scence of Tb(III). Excitation is at 290 nm and emission wavelengths ar e 490 nm and 545 nm. A linear relation is obtained between the fluores cence intensity and dGMP concentration in the range 2.0 x 10(-7) to 1. 0 x 10(-5) mol l(-1). The detection limit is 3.0 x 10(-8) mol l(-1). T his method can determine dGMP selectively in the presence of other nuc leotides (not including GMP).