ARMILLARIA SPECIES OF THE OLYMPIC PENINSULA OF WASHINGTON-STATE, INCLUDING CONFIRMATION OF NORTH-AMERICAN BIOLOGICAL SPECIES-XI

Forty-eight collections of Armillaria from the Olympic Peninsula of Wa shington State were identified to species using the conventional pairi ng protocol and/or a polymerase chain reaction-based (PCR) technique u tilizing restriction fragment polymorphisms (RFLPs) of the intergenic spacer region (IGS) of the ribosomal DNA. The PCR-based technique yiel ded results comparable to those obtained by the conventional pairings. Several restriction patterns not previously reported in North America n isolates were found using the enzyme Alu I. Collections from eight d ifferent host species were identified as A. ostoyae, A. sinapina, A. n absnona, A. gallica, or North American biological species (NABS) XI. A rmillaria ostoyae, A. sinapina, and A. nabsnona were the most often co llected, with the first two occurring predominantly on gymnosperms and the latter mostly on angiosperms. Armillaria gallica and NABS XI were collected rarely and only from angiosperms. This is only the second r eport of the occurrence of NABS XI.