INCREASED EXPRESSION OF GROWTH-ASSOCIATED PROTEIN-43 IMMUNOREACTIVITYIN AXONS FOLLOWING COMPRESSION TRAUMA TO RAT SPINAL-CORD

Growth-associated protein 43 (GAP43) is one compound used to indicate growth of axonal endings during development and regeneration, particul arly of peripheral neurons. Using immunohistochemistry, we have studie d the expression of GAP43 in the spinal cord of rats subjected to mild , moderate or severe compression injury and used neurofilament immunos taining to demonstrate axonal injuries. Samples removed from the compr essed T8-9, the cranial T7 and the caudal T10 segments were studied at 4 h, 24 h, 4 days and 9 days after injury. Control rats showed a mode rate immunostaining of neurons in dorsal root ganglia, weak staining o f ventral motor neurons and, with the exception of the corticospinal t racts, a weak staining in some axons of the longitudinal tracts of the cord. Injury in the compressed region led to increased GAP43 immunore activity in axons of normal and expanded size. This occurred particula rly 1-4 days after injury and normalized 9 days thereafter. More marke d immunostaining was present in the cranial and caudal segments. The c orticospinal tracts never showed such staining. The increase of GAP43 immunostaining is presumably caused by disturbed axonal transport from neurons with the capacity to synthesize and transport the GAP43 antig en. Transported material may thus be available for regeneration of axo ns, but this source of material may vary between different classes of axons within the cord.