INFLUENCE OF EXTRACTION SOLVENT AND TEMPERATURE ON THE QUANTITATIVE-DETERMINATION OF OLIGOSACCHARIDES FROM PLANT MATERIALS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
Hn. Johansen et al., INFLUENCE OF EXTRACTION SOLVENT AND TEMPERATURE ON THE QUANTITATIVE-DETERMINATION OF OLIGOSACCHARIDES FROM PLANT MATERIALS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of agricultural and food chemistry, 44(6), 1996, pp. 1470-1474
The effect of extraction solvents and temperatures on extraction yield
s of monosaccharides, sucrose, and raffinose oligosaccharides from pla
nt materials was investigated. Toasted soybean meal, cotton seed meal,
field peas, and a feed mixture were extracted in either water, 50% (v
/v), or 80% (v/v) aqueous methanol or ethanol at 20 or 50 degrees C or
at the boiling point of the solvent. Extraction in 80% (v/v) alcohol
was strongly influenced by the extraction temperature and maximum extr
action was only achieved at the boiling point. Extraction in water and
50% (v/v) methanol or ethanol was less heat sensitive and gave compar
able results. Aqueous ethanol (50%, v/v) was as effective as 50% (v/v)
methanol, whereas lower yields were seen at higher alcohol strength.
There was no consistent difference in the extraction yield when compar
ing reflux with constant stirring and water bath with occasional mixin
g for any of the extraction solvents used.