Bt. Ariyo et al., ALGINATE OLIGOSACCHARIDES AS ENHANCERS OF PENICILLIN PRODUCTION IN CULTURES OF PENICILLIUM-CHRYSOGENUM, Biotechnology and bioengineering, 53(1), 1997, pp. 17-20
Oligosaccharide fragments were prepared by partial acid hydrolysis of
sodium alginate and consisted of oligomannuronate (OM) and oligoguluro
nate (OG) blocks. Effects of the OM and OG blocks on penicillin G prod
uction by P. chrysogenum were investigated. The oligosaccharides were
found to cause significant increases in penicillin G yields. OM blocks
at concentrations 10 to 100 mu g/mL were used to further evaluate the
effects of the oligosaccharides, and were found to enhance the produc
tion of penicillin G in shaken flask cultures of P. chrysogenum P2 (hi
gh penicillin producer) and NRRL 1951 (low penicillin producer) at the
test concentrations. There was an approximately 50% maximum increase
in penicillin G yield from biomass in P. chrysogenum P2 cultures and 1
50% in P. chrysogenum NRRL 1951 cultures, when compared to control cul
tures without the oligosaccharides. (C) 1997 John Wiley & Sons, Inc.